Activity of European sea bass leucocytes against nodavirus- infected cells: a functional and RNA-seq study
Chaves-Pozo, E. (Elena); Valero, Y. (Yulema); Esteve-Codina, A. (Anna); Gómez-Garrido, J. (Jèssica); Dabad, M. (Marc); Alioto, T. (Tyler); Meseguer, J. (José); Esteban, M.A. (M.Ángeles); Cuesta, A. (Alberto)
Seabream HKLs showed significantly increased innate CMC activity against NNV-infected cells, compared to those uninfected, while sea bass HKLs failed to do so. Thus, we performed a RNA-seq study to identify genes related to the CMC activity of sea bass leucocytes. Thus, we found that sea bass HKLs incubated with DLB-1 cells alone (CMC_DLB1) or with NNV-infected DLB-1 cells (CMC_DLB1-NNV) showed very similar transcriptomic profiles and the GO analysis revealed that most of the up-regulated genes were related to immunity. Strikingly, when the CMC samples with and without NNV were compared, GO analysis revealed that most of the up-regulated genes in CMC_DLB1-NNV samples were related to metabolism and very few to immunity. This is also in agreement with the functional data. These data point to the escape of CMC activity by NNV infection as an important factor involved in the high susceptibility to nodavirus infections of European sea bass.
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